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Specifications
- Host Species
- Mouse
- Antibody Isotype
- IgG2b
- Clone
- GH5S
- Species Reactivity
- N/A
- Immunogen
- DYKDDDDK (FLAG) synthetic peptide conjugated to KLH.
- Conjugate
- Unconjugated
Applications
- Application Notes
- WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature); ICC: 1:500-2000 dilution
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Target
- Alternative Names
- FLAG Tag; FLAG-tag; FLAG octapeptide; DDDDK epitope tag; DYKDDDDK epitope tag; ECS epitope tag; ECS tag; Enterokinase Cleavage Site epitope tag; flag
References
Zhang, RD; Kim, TK; et al. Biochemical and mass spectrometric characterization of the human CB2 cannabinoid receptor expressed in Pichia pastoris - Importance of correct processing of the N-terminus. PROTEIN EXPRESSION AND PURIFICATION 55:225-235(2007).
Go, YY; Snijder, EJ; et al. Characterization of Equine Humoral Antibody Response to the Nonstructural Proteins of Equine Arteritis Virus. CLINICAL AND VACCINE IMMUNOLOGY 18:268-279(2011).