陈涛‘，孙妮z，王艳3
c1华中农业大学食品科技学院，湖北武汉430070; 2中国科学院南海海洋研究所，广东广州510301;
3中国科学院烟台海岸带研究所，山东烟台264003)
摘要:口的:以小球藻Chlorella zofi}agie}a.si.s为研究对象，以葡萄糖和木糖为混合碳源，优化混合碳源浓度提高异养培养虾青素
产量。方法:利用JMP软件中完全析因设训一法考查葡萄糖和木糖浓度对虾青素产量的影响，并采用SAS软件对实验数据进行回
归分析。结果:优化条件下葡萄糖和木糖浓度分别为26. 86 g/L和16.92 g/L。结论:优化后的虾青素产量达到8. 65 mg/L，比初
始条件产量7. 39 mg/L提高了17% o
关键词:小球藻;葡萄糖;木糖;异养;优化;虾青素
中图分类号:'fQ920. 6文献标识码:A doi:10.3969/j.issn.1004-311X.2013.03.0073
Effects of Glucose and Xylose on the Production of
Astaxanthin by Chlorella zofingiensis in the Dark
CHEN Tao'，SUN NiZ，WANG Yan;
(1. College of food Science and Technology, lluazhong Agricultural University, U'uhan 430070, China;
2. South China Sea lnstitute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301，China;
3. Yantai lnstitute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China)
Abstract : O衍ective:Optimization of the c;onc;entration of glucose and xylose was carried out to increase astaxanthin yield by Chlorella:州二
i}agie}a.si,s heterotrophic;ally. Method:Full Factorial Design by software JMP was used to investigate the elfec;ts of glucose and xylose nn the
production of astaxanthin, and the experimental data was analyzed using regression analysis method by software SAS. Result: The optimal
c;onc;entrations of glucose and xylose were 26. 86 g/L and 16. 92 g/L. Conclusion:Astaxanthin yield of C. z听}agie}a.si,s could reach 8. 65
mg/L under optimum c;onc;entration, which was 17% higher than that achieved under original c;onc;entration (7. 39 mg/L)
Key words:Chlorella:听ttgiett,si,s;Glucose;Xylose;Heterotrophic;ally;Optimization;Astaxanthin
