工程化酿酒酵母合成植物三萜类化合物
朱明‘，王彩霞z，李春‘,z
(‘天津大学化工学院，系统生物工程教育部重点实验室，天津300072;北京理工大学生命学院，北京100081
摘要:三萜类化合物如甘草次酸、皂苷等是许多药物在细胞内发挥药理活性的主要存在形式，可作为药物的主要
活性成分，有些还可作为甜味剂等。但是萜类化合物在天然植株中含量很低，小能很好地对其开发和利用。随着
萜类物质代谢中关键酶的发现，整个萜类代谢途径变得清晰。近年来合成生物学快速发展，为利用微生物发酵生
产三萜化合物奠定了基础。综述了酿酒酵母中三萜化合物的合成途径及在此途径中起重要作用的细胞色素单氧化
酶的研究进展。
关键词:三萜类化合物;皂苷;刀一香树脂醇;CYP450氧化酶;合成生物学
DOI: 10.11949/j.issn.0438-1157.20150914
中图分类号:Q 819文献标志码:A文章编号:0438-1157 <2015) 09-3350-07
Engineered Saccharomyces cerevisiae for biosynthesis of plant triterpenoids
ZHU Mingl, WANG Caixia2, LI Chunl z
(1Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering, Tianjin University,
Tianjin 300072, China; 'School of Life Science, Beijing Institute of Technology, Beijing 100081, China)
Abstract: Triterpenoids (such as glycyrrhetinic acid, saponin)is the main existence form of many drugs that play
a pharmacological activities within the cells. Except used as the main active ingredients of drugs, some of them
also can be used as a sweetener. But the amount of triterpenoids in plant (the origin source) is very low, which
restrict its development and utilization. With the discovery of key enzymes in terpenoids metabolism, the whole
metabolic pathway of terpenoids is clear increasingly. In recent years, the rapid development of synthetic biology
lays a solid foundation for triterpenoids production by microbial fermentation. This paper reviews the triterpenoids
biosynthesis pathway in Saccharomyces cerevisiae and cytochrome P450 monooxygenase (CYP450) which plays
an important role in the whole pathway.
Key words: triterpenoids; saponin;刀-amyrin; cytochrome P450 monooxygenase; synthetic biology
