陈小燕，许敬亮，袁振宏†，梁翠谊，张 宇
（中国科学院广州能源研究所，中国科学院可再生能源重点实验室，广州 510640）
摘 要：利用高温酵母进行高温乙醇发酵，因其能量损耗低、发酵速度快、染菌机率低等优点，成为生物质乙醇发
酵生产的新方向。相比传统酵母，马克斯克鲁维高温酵母具有良好的耐温适应能力、更宽泛的底物利用能力、良好
的发酵性能、超强的分泌能力、适于分子生物学操作等优势，在生物质乙醇发酵方面显示巨大潜力。本文从底物利
用能力、基因重组方法、发酵方法等方面总结了马克斯克鲁维酵母在生物质乙醇发酵方面的发展概况。
关键词：马克斯克鲁维酵母；生物质乙醇；菌株改造
中图分类号：TK6 文献标志码：A doi：10.3969/j.issn.2095-560X.2014.05.007
Studies in Biomass Ethanol Production by Kluyveromyces marxianus
CHEN Xiao-yan, XU Jing-liang, YUAN Zhen-hong, LIANG Cui-yi, ZHANG Yu
(CAS Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion,
Chinese Academy of Sciences, Guangzhou 510640, China)
Abstract: With the advantages of low energy-cost, rapid fermentative rate, and less contaminant probabilities, ethanol
fermentation at high-temperature has becomes the emerging preference for biofuel production. Compared to conventional
yeast, Kluyveromyces marxianus shows the abilities of wider substrate utilization, favorable fermentative performance,
powerful capability of protein secretion, suitable for molecular manipulation, which is potential for bio-ethanol production.
This paper reviewed the advances of Kluyveromyces marxianus in the field of biomass ethanol production.
Key words: Kluyveromyces marxianus; biomass ethanol; DNA recombinant technology
