Anti-FLAG tag monoclonal antibody (DMAB8054)

Host Species

Mouse

Antibody Isotype

IgG2b

Clone

GH5S

Species Reactivity

N/A

Immunogen

DYKDDDDK (FLAG) synthetic peptide conjugated to KLH.

Conjugate

Unconjugated

Application Notes

WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature); ICC: 1:500-2000 dilution

*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Alternative Names

FLAG Tag; FLAG-tag; FLAG octapeptide; DDDDK epitope tag; DYKDDDDK epitope tag; ECS epitope tag; ECS tag; Enterokinase Cleavage Site epitope tag; flag

Zhang, RD; Kim, TK; et al. Biochemical and mass spectrometric characterization of the human CB2 cannabinoid receptor expressed in Pichia pastoris - Importance of correct processing of the N-terminus. PROTEIN EXPRESSION AND PURIFICATION 55:225-235(2007).

Go, YY; Snijder, EJ; et al. Characterization of Equine Humoral Antibody Response to the Nonstructural Proteins of Equine Arteritis Virus. CLINICAL AND VACCINE IMMUNOLOGY 18:268-279(2011).